Microscopy
BASIC DATA
course listing
A - main register
course code
LTK9000
course title in Estonian
Mikroskoopia
course title in English
Microscopy
course volume CP
-
ECTS credits
3.00
to be declared
yes
fully online course
not
assessment form
Examination
teaching semester
spring
language of instruction
Estonian
English
Study programmes that contain the course
code of the study programme version
course compulsory
no
Structural units teaching the course
LT - Department of Cybernetics
Timetable link
Version:
VERSION SPECIFIC DATA
course aims in Estonian
Kursuse eesmärk on anda nii teoreetiline kui ka praktiline ülevaade mikroskoopiast ja selle rakendustest bioloogias.
course aims in English
The aim of the course is to provide both theoretical and practical overview of microscopy and its applications in biology.
learning outcomes in the course in Est.
Õppeaine läbinud üliõpilane:
- kirjeldab mikroskoopia põhiprintsiipe;
- tuvastab uurimistööks sobilikud meetodid ning kasutab neid püstitatud hüpoteeside testimiseks;
- rakendab omandatud teadmisi ja oskusi oma uurimistöös.
- kirjeldab mikroskoopia põhiprintsiipe;
- tuvastab uurimistööks sobilikud meetodid ning kasutab neid püstitatud hüpoteeside testimiseks;
- rakendab omandatud teadmisi ja oskusi oma uurimistöös.
learning outcomes in the course in Eng.
After completing this course, the student:
- describes the basic principles of microscopy,
- identifies suitable microscopy methods for a specific research questions and uses them to test the hypotheses;
- applies the acquired knowledge and skills to her/his own research.
- describes the basic principles of microscopy,
- identifies suitable microscopy methods for a specific research questions and uses them to test the hypotheses;
- applies the acquired knowledge and skills to her/his own research.
brief description of the course in Estonian
Kursuse raames antakse seminaride vormis ülevaade erinevatest mikroskoopia meetoditest ja põhiprintsiipidest. Sissejuhatus mikroskoopiasse; fluorestsentsmikroskoopia; konfokaalmikroskoobi tööpõhimõtted. Konfokaalmikroskoopia rakendamine bioloogilistele preparaatidele; autofluorestsents, fluorestsentsvärvained, fluorofoorid. Interferentsi filtrid konfokaalmikroskoopias. Laserid konfokaalmikroskoopias. Konfokaalmikroskoobi objektiivid ja skanneerimissüsteemid. Fluorestsentskorrelatsiooni spektroskoopia. Tarkvara mikroskoopia piltide analüüsiks.
brief description of the course in English
The course provides an overview of the basic principles of microscopy in the form of seminars. Introduction to microscopy; fluorescence excitation and emission fundamentals; principles of confocal microscopy. Applying confocal microscopy to biological specimens; autofluorescence, fluorescence dyes, fluorophores. Interference filters for confocal microscopy. Spectral bleed-through artifacts in confocal microscopy. Laser systems in confocal microscopy. Confocal microscope objectives and scanning systems. Fluorescence correlation spectroscopy. Software for microscopy image analysis.
type of assessment in Estonian
-
type of assessment in English
-
independent study in Estonian
-
independent study in English
-
study literature
Handbook of Biological Confocal Microscopy: 3rd (Third) edition; ed JB Pawley
study forms and load
daytime study: weekly hours
2.0
session-based study work load (in a semester):
lectures
1.0
lectures
-
practices
1.0
practices
-
exercises
0.0
exercises
-
lecturer in charge
-
LECTURER SYLLABUS INFO
semester of studies
teaching lecturer / unit
language of instruction
Extended syllabus
2024/2025 spring